Objective:To explore the possible mechanism of improved insulin resistance in the rat model of GDM after the intervention of dietary PUFA.Methods:The rat model of GDM was established by streptozotocin(STZ).Thirty of them were used and randomized evenly divided into three groups:control group (STZ,n =10),Group 1 (STZ +low calorie,n =10) and Group 2(STZ + low calorie + PUFA,n =10).Crude membrane,plasma membrane and intracellular membrane fractions of adipocyte were isolated by different speed of centrifugations.The relative amount of GLUT in membrane fractions was detected by D-[U-14C]-glucose radioactivity.Results:The amount of GLUT in membrane fractions in Group 1 and Group 2 were significantly higher than that in Control Group (P＜0.01),while GLUT in Group 2 was markedly higher than Group 1.Conclusions:PUFA diet increase the amount of GLUT in adipocyte,which may be a segment of ameliorating insulin resistance in GDM rats.%目的:探讨膳食多不饱和脂肪酸(PUFA)改善糖尿病孕鼠脂肪细胞中胰岛素抵抗的可能机制.方法:利用链脲佐菌素(STZ)建立妊娠期糖尿病孕鼠模型,取30只模型鼠随机分成对照组、干预组1(低热卡饮食)和干预组2(低热卡饮食+ PUFA),每组10只.分离孕鼠脂肪细胞粗膜、内膜、外膜；利用D-(U-14C)-葡萄糖分别测定其葡萄糖转载体(GLUT)相对含量.结果:干预组1和干预组2孕鼠脂肪细胞粗膜、内膜、外膜的GLUT含量均高于对照组(P＜0.01),干预组2孕鼠脂肪细胞粗膜、内膜、外膜的GLUT含量高于干预组1(P＜0.01).结论:PUFA饮食增加妊娠期糖尿病孕鼠脂肪细胞的GLUT,PUFA可能是改善胰岛素抵抗的环节之一.
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