目的:评价马尾松树皮提取物(PMBE)和葡萄籽提取物(GSE)对根面牙本质耐酸蚀脱矿作用的影响.方法:表面一半被覆盖的根面牙本质块40个,被随机分为4组(n=10),分别用去离子蒸馏水(DDW)溶液、0.1％NaF溶液、12％ PMBE溶液和12％ GSE溶液作为实验溶液,进行为期8d的pH循环(实验溶液,酸性缓冲液和中性缓冲液).显微CT测定各组未脱矿侧和脱矿侧牙本质的矿物密度(dentin mineral density,DMD),场发射扫描电镜观察各组pH循环后牙本质表面显微形貌.结果:牙本质的矿物密度显示DDW组脱矿侧和未脱矿侧牙本质矿物密度差(△DMD) 198.64±59.97,NaF组为45.94±24.21,PMBE组为90.23±28.77,GSE组为105.07±29.53.PMBE组和GSE组△DMD均高于NaF组(P＜0.05),低于DDW组(P＜0.05),两者相互之间无显著差异(P＞0.05).扫描电镜见DDW组牙本质表面牙本质小管完全开放,NaF组牙本质小管基本封闭,PMBE组和GSE组牙本质小管口成梭形或狭长微裂隙开口.结论:PMBE和GSE均能提高牙本质的耐酸蚀脱矿作用.%Objective:To evaluate the effect of pinus massoniana bark extract (PMBE) and grape seed extract (GSE) on dentin demineralization caused by acid.Methods:40 root dentin blocks with half of the surface covered were randondy divided into 4 groups (n =10).All samples were subjected to pH cycling for 8 days,and deionized distilled water(DDW),0.1％NaF,12％ PMBE solution and 12％ GSE solution were used as the experimental solutions in the 4 groups.The dentin mineral density(DMD) of the both sides was determined using micro-computed tomography.The D-value of DMD between nn-demineralized and demineralized side (△DMD)was calculated.The samples were observed with field emission scanning electron microscops (FE-SEM).Results:The △DMD of DDW,NaF,PMBE and GSE groups was 198.64 ±59.97,45.94 ±24.21,90.23 ±28.77 and 105.07 ±29.53 respectively.The △DMD between PMBE and GSE groups had no significant difference (P ＞ 0.05),which were both higher than that of NaF group (P ＜ 0.05) and lower than that of DDW group(P ＜ 0.05).The FE-SEM revealed that the dentin tubules in DDW group were completely open,but in NaF group were essentially closed in PMBE group and GSE group were spindle shaped or narrow crack opening.Conclusion:PMBE and GSE had almost the same effect on improving the acid resistance of dentin.
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