Objective:To explore the effects of radiation on miRNA-21 expression and the biology of human salivary gland adenoid cystic carcinoma cell lines(SACC-83,SACC-LM).Methods:In vitro cultured SACC-83 and SACC-LM cells were radiated by Varian 23 EX with the dose of 0(control),2,6 and 10 Gy respectively.Cell proliferation,apoptosis and miRNA-21 expression were observed by CCK-8 assay,flow cytometry and qRT-PCR methods respectively.Results:48 h after radiation the proliferation ability of SACC-LM cells decreased with the increasing dose of radiation(F =1 321.646,P =0.000),so did the miRNA-21 expression (F =177.964,P =0.011).However,the early apoptosis rate (F =354.484,P =0.039),the later apoptosis rate (F =254.278,P =0.042) and heteroploid ratio(F =1 562.991,P =0.001) increased with the increasing dose of radiation.In SACC-83 cell line 48 h after 6 Gy radiation the cell proliferation(F =1 537.214,P =0.013) and the miRNA-21 expression(F =134.868,P =0.017) were lower than that of other radiation doses.Moreover,the early apoptosis rate (F =88.579,P =0.006),the late apoptosis rate (F =1 391.345,P =0.033),heteroploid ratio(F =250.461,P =0.004) were higher than that of other radiation doses.Conclusion:The miRNA-21 expression in SACC-LM and SACC-83 cell lines is conversely associated with the radiation sensitivity.%目的:研究放疗对人涎腺腺样囊性癌细胞SACC-83和SACC-LM的影响以及与miRNA-21表达的相关性.方法:用Varian 23 EX对培养中的SACC-83和SACC-LM予从0(对照)、2、6、10 Gy照射,CCK-8检测、流式细胞仪观察放射处理后SACC-83、SACC-LM的细胞增殖和凋亡的变化;使用qRT-PCR法分析不同剂量放疗后SACC-83、SACC-LM细胞miRNA-21的表达变化.结果:SACC-LM细胞放射处理48 h后增殖能力随放疗剂量的增大而降低(F=1 321.646,P=0.000),早期凋亡率(F=354.484,P=0.039)、晚期凋亡率(F=254.278,P=0.042)和异倍体比例(F=1 562.991,P=0.001)随着放疗剂量的增大而升高,且miRNA-21的相对表达量随放疗剂量的增大而减小(F=177.964,P=0.011);SACC-83细胞放疗48 h后6 Gy放疗组细胞增殖能力明显低于其他各放疗组(F=1 537.214,P=0.013),6Gy剂量下早期凋亡率(F=88.579,P=0.006)、晚期凋亡率(F=1 391.345,P=0.033)、异倍体比例(F=250.461,P=0.004)最高,miRNA-21的相对表达量最低(F=134.868,P=0.017).结论:miRNA-21可能与SACC-LM、SACC-83细胞的放疗敏感性呈负相关.
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