Objective:To investigate the migration and odontogenesis of rat dental pulp cells transfected with adenovirus vector enco-ding cadherin-11.Methods:The dental pulp cells were cultured and transfected with pDC316-mCMV-EGFP-Cadherin-11.Cad-11 protein expression of the cells was examined by immunohistochemistry staining.The odontogenic differentiation of the cells was studied by alizarin red staining and ALP staining.The adhesion and migration of the cells were tested.Results:Transfection of pDC316-mC-MV-EGFP-Cadherin11 promoted Cad-11 protein expression and ALP activity,increased calcified nodule formation(P <0.05),adhe-sion and migration of rat dental pulp cells(P <0.05).Conclusion:Cadherin-11 may promote the odontoblast differentiation and mi-gration of rat dental pulp cells.%目的：探讨腺病毒载体介导的 Cadherin-11（Cad-11）基因转染对大鼠牙髓细胞迁移能力和成牙本质分化能力的影响。方法：原代培养大鼠牙髓细胞，并用 pDC316-mCMV-EGFP-Cadherin11重组腺病毒进行转染。通过免疫组化法检测 Cad-11蛋白表达，用 ALP 染色和茜素红钙结节染色及定量检测其成牙本质分化情况。黏附实验和迁移实验分别检测 Cad-11对牙髓细胞黏附和迁移的影响。结果：免疫组化染色示 Cad-11转染成功并高表达 Cad-11蛋白。Cad-11在矿化诱导14 d 时可增强大鼠牙髓细胞 ALP 表达，诱导21 d，形成钙化结节量增加（P ＜0．05），同时增加细胞的黏附性和迁移能力（P ＜0．05）。结论：Cad-11能促进大鼠牙髓细胞成牙本质分化能力，同时提高其迁移活性。
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