目的:探讨富血小板纤维蛋白提取液(PRFe)对成骨细胞增殖、分化及细胞骨架的影响.方法:将培养中的小鼠成骨细胞MC3T3-E1分为2组,实验组采用50％浓度PRFe,对照组为正常αt-MEM培养液.MTr法检测细胞增殖；碱性磷酸酶(ALP)试剂盒检测ALP活性；茜素红染色观察细胞矿化功能,并用图像分析软件进行半定量分析；激光共聚焦显微镜观察细胞骨架形态.结果:MTT实验显示随时间延长,细胞数目明显增加(P＜0.05),在各时间点,实验组细胞数量明显高于对照组(P＜0.05)；ALP检测显示随时间延长,ALP活性明显增大(P＜0.05),在各时间点,实验组A值均显著大于对照组(P＜0.05)；茜素红染色显示随时间延长,钙结节染色的积分吸光度值逐渐增大(P＜0.05),每一时间点,实验组的钙结节积分吸光度值大于对照组(P＜0.05)；细胞骨架观察显示在各时间点,实验组细胞骨架较对照组更加伸展.结论:PRFe能促进MC3T3-E1细胞的增殖、分化,对细胞骨架的排列和伸展有促进作用.%Objective:To evaluate the effects of platelet-rich fibrin extract (PRFe) on the proliferation and differentiation of osteoblast MC3T3-E1 cells.Methods:MC3T3-E1 cells were cultured in 50％ PRFe (test group) and normal α-MEM respectively (controlgroup).The proliferation,alkaline phosphatase (ALP) activity and mineralization were examined by MTT assay,ALP Kit and Alizarin red dye staining respectively; the F-actin cytoskeleton was observed by confocal laser scaning microscopy (CLSM).Results:PRFe treatment increased the proliferation(P ＜ 0.05),ALP activity(P ＜ 0.05),and calcium nodus formation of MC3T3-E1 cells(P ＜0.05) in a time-dependant manner.At each time point,filaments in PRFe treated cells were more well spread than those in the untreated.Conclusion:PRFe may stimulate the proliferation and differentiation of osteoblasts and can promote the spread of F-actin cytoskeleton.
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