目的：利用Periocheck椅旁快速检测观察牙周治疗前后龈下菌斑肽酶活性的改变及其与牙周临床指标的相关性。方法：利用Periocheck椅旁快速检测65例牙周炎患者共65个位点洁治前后龈下菌斑中牙龈卟啉菌、福塞类杆菌、齿垢密螺旋体来源的肽酶活性的改变，通过与标准比色卡比较区分阳性和阴性；在666 nm下测吸光度值，定量反映肽酶活性。结果：治疗前牙周炎位点肽酶活性(0.44±0.23)明显高于治疗后(0.34±0.26)，差异有显著性(P<0.05)；治疗后Periocheck(+)部位牙周袋深、附着丧失、牙龈指数和Periocheck(-)位点相差显著。结论：Periocheck方法能够快速、简便、灵敏的检测龈下菌斑中肽酶活性，反映牙周临床指标的改变，用于牙周治疗效果的监测。%Objective:To evaluate the usefulness of Periocheck in clinical application before and after periodontal curettage.Methods:Periocheck was applied in 65 sites in 65 adult patients with periodontitis within 15 min before and after scaling and root planing to monitor peptidase activity produced by Porphyromones gingivalis (Pg),Treponema denticola(Td) and Bacterides forsythus (Bf) in subgingival plaque. The peptidase activity was determined both spectrophotometrically and by using color standard.Results:The peptidase concentrations (Try U/L) before and after treatment were 0.44±0.23 and 0.34±0.26 (P<0.05),while Periocheck positiveness was found in 65/65 and 43/65 (P<0.01) of the patients,respectively.After treatment clinical parameters such as GI, PD,AL and peptidase concentration decreased (P<0.05) in 22 Periocheck positive patients,while those did not (P>0.05) in 43 negitive.Conclusion:Periocheck might provide a promising chair-side monitoring tool for the evaluation of scaling and root planing.
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