目的：分析分子外科缝线法基因转染的安全性。方法：将1 cm长外科缝线浸泡于含pBLacZ质粒的PBS中过夜，然后将其移植在兔舌肌内。针对转入体内的外源性基因DNA有可能整合到宿主细胞染色体上引起细胞生长行为改变的潜在危险性，采用流式细胞仪技术对实验组和对照组的细胞增殖动力学进行了检测；针对基因免疫有可能引起抗质粒双链DNA抗体产生的潜在危险性，采用间接免疫荧光法对基因转染前后兔血清中的抗dsDNA抗体进行了检测。结果：转染前后无抗-dsDNA抗体产生，实验组和对照组FCM检测无改变。结论：分子外科缝线术转染外源基因的方法不会引起细胞生长行为的改变和诱发抗双链DNA抗体的产生，是一种安全的基因转染方法。%Objective:To study the safety of gene transfection with surgical suture into rabbit's tongue muscle .Methods:pBLacZ gene was transfected into tongue muscle of rabbit by implantation of 1 cm surgical suture,which had been soaked in pBlacZ plasmid solution for overnight.The growth of the muscle cells was analyzed by flow cytometry.The adverse production of anti-double strand DNA was detected by indirect immunofluorescent assay.Result:No difference was found in the growth between gene transfected muscle cells and the controls.No anti-double strand DNA antibody was detected .Conclusion:This gene transfer system may not induce growth change and anti-double strand DNA antibody in the transfected cells.
展开▼
