目的：探讨TGF-β1 和口腔鳞癌侵袭转移的关系。方法：采用发色底物反应法测量不同浓度TGF-β1 作用于口腔鳞癌TSCCa 细胞系及颈淋巴转移癌GNM 细胞系u-PA 和PAI-1 的活性。结果：不同浓度的TGF-β1 作用于TSCCa 12 h 后，u-PA 和PAI-1 活性与对照组相比，无明显差异(P>0.05)；而不同浓度的TGF-β1 作用于GNM 细胞12 h 后，u-PA和PAI-1 活性与对照组相比，有显著性差异(P<0.05～0.01)，u-PA和PAI-1 活性与TGF-β1 有剂量依赖关系。结论：TGF-β1 可能与口腔鳞癌侵袭转移有关。%Objective:To study the role of transforming growth factor β1(TGF-β1) on the invasiveness and metastasis of human oral squamous cell carcinoma (OSCC).Methods:Chromogenic substrate assay was used to determine the activity of urokinase plasminogen activator(u-PA) and plasminogen activator inhibitor-1(PAI-1) in OSCC cell line TSCCa and metastatic carcinoma cell line GNM treated with various concentrations of TGF-β1.Results:TGF-β1 at 0.5～5.0 ng/L had no effect on the activity of u-PA and PAI-1 in TSCCa cells (P>0.05);while TGF-β1 at the same concerntrations increased the activity of u-PA and PAI-1 in GNM cells (P<0.05).Conclusion:TGF-β1 may be a factor associated with invasive and metastatic potential of OSCC.
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