Objective The objectives were to know knowledge-attitude–practice level with Standard Precaution among medical staffs in Shenzhen first-class hospitals.Methods Totally 170 medical staffs were investigated with a self designed questionnaire using stratiifed random sampling.Results Totally 170 valid questionnaires (54 doctors and 116 nurses) were collected. There was statistical significance in profession, working years, professional title and degree about know knowledge-attitude–practice level with Standard Precaution (P<0.01). It is influenced by many factors. The signiifcant differences between doctors (18.52%) and nurses (58.62%) has been found in hand hygiene. Medical staff who always "masks" and "wear isolation gowns" is just 43.53% and 23.53%. The main reason is "time" (35.29%) and "protective equipment supply" (36.47%). 88.24% medical staffs have been injured by sharp instrument, but 78.89%medical staffs do not report. Because 55.17% think the procedure is complex.Conclusion It is necessary to strengthen medical staffs’ training, correct errors, and increase compliance with standard precaution.%目的：了解三甲医院口腔门诊医护人员标准预防认知现状，防治医院感染的发生。方法采用随机抽样的方法，自行设计问卷，对170名口腔门诊医护人员进行调查。结果共回收有效问卷170份，其中，医生54份，护士116份。职业，工龄，职称，学历对标准预防相关知识了解的差异具有统计学意义(P<0.01)；标准预防依从性受多种因素的影响。其中手卫生的依从性护士为58.62％，医生仅有18.52%，医护人员间的差异具有统计学意义。而影响手卫生依从性的主要因素为“工作忙碌/无足够时间”及“需优先处理病人”(58.83%，36.47%)。总是“戴面罩”和“穿隔离衣”的医护人员仅43.53%，23.53%。其主要原因是“时间紧迫”与“防护设备供应不足”(35.29%，36.47%)。曾锐器损伤的占总人数的88.24%，而高达78.89%的人选择不上报。众多不上报的原因中，55.17%的人认为上报程序复杂。结论应加强对口腔门诊医护人员标准预防相关知识的培训，纠正对标准预防错误的认识，提高标准预防的依从性十分必要。
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