Objective: To examine the potential effect of applying different concentration of extracellular trehalose on mouse oocyte vitrification.rnMethods; The recover rate of mouse oocytes was detected as a functional index of different extracellular trehalose concentrations. A total of 360 oocytes were obtained from 12 superstimulated mice and cultured for 3 hours in freezing medium added 0, 0. 3, 0. 5, and 1. 0 mol/L trehalose. Forty oocytes were stained with propidium iodide (PI). Three hundreds and twenty oocytes were vitrified, warmed and tested the developmental potential after in vitro fertilization and culture.rnResults: There was no PI staining positive cell among the four groups. The fertilization rate and recover rate in 0. 3 mol/L trehalose group were not significantly different with control group(92. 59% vs. 90.90%, 70.67% vs. 61. 43%)(P>0. 05). The cleavage rate and blastocyst rate in the 0. 3 mol/L group were significantly higher than those in the control group(83. 02% vs. 44.19%, 72. 72% vs. 47.37%)(F< 0.001). The recover rate, fertilization rate and blastocyst rate in 0. 5 mol/L trehalose group were not significantly different with those in control group (P>0. 05), but the cleavage rate in 0. 5 mol/L group was significantly higher than that in control group (65. 22% vs. 44. 19%) (P<0. 05). The blastocyst rate in 0. 3 mol/L group was significantly higher than that in 0. 5 mol/L group(72. 72% vs. 50. 00%) (P< 0.001).Conclusions: Certain amount of trehalose had protective effect on the vitrification oocyte of mouse. A concentration of 0. 3 mol/L trehalose was more suitable for oocytes vitrification in mouse.%目的 探讨海藻糖对小鼠卵母细胞玻璃化冷冻的影响. 方法 采用12只小鼠控制性超排卵获得的360枚成熟的卵母细胞,分别放入含1.0、0.5、0.3及0 mol/L的海藻糖中孵育3h.部分卵母细胞进行碘化丙啶(PI)染色；320枚卵母细胞进行玻璃化冷冻和复苏,并进行体外受精,观察卵母细胞成活率、受精率、卵裂率及囊胚形成率. 结果 0.3 mol/L海藻糖组复苏率、受精率与对照组无显著差异(92.59％ vs.90.90％,70.67％ vs.61.43％),但是卵裂率、囊胚形成率均高于对照组(83.02％ vs.44.19％,72.72％ vs.47.37％)(P＜0.001)；0.5 mol/L海藻糖组复苏率、受精率、囊胚形成率与对照组无显著差异,但是卵裂率高于对照组(65.22％ vs.44.19％)(P＜0.05)；0.3 mol/L海藻糖组囊胚形成率高于0.5 mol/L海藻糖组(72.72％ vs.50.00％)(P＜0.001);1.0 mol/L海藻糖组卵母细胞复苏率、受精率、卵裂率、囊胚形成率均低于对照组低(P＜0.001). 结论 一定浓度范围的海藻糖对小鼠卵母细胞的玻璃化冷冻有保护作用,0.3 mol/L海藻糖浓度比较适合小鼠卵母细胞玻璃化冷冻.
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