Objective]1 547 suspected infected tobacco samples from 36 cities / counties in Guizhou were tested to identify major species of viruses in the tobacco growing area.[Method]DAS-ELISA testing process was used to analyze these samples.[Results]1 107 samples were de-tected to be positive infection.They included five kinds of viruses:tobacco mosaic virus,potato virus Y,cucumber mosaic virus,tobacco etch virus and tobacco vein banding mosaic virus.TMV and PVY have a high detection rate,which were 41. 63% and 11. 70%,respectively.The major way of infection was single virus infection.Besides,four mixed infection forms were also detec-ted.[Conclusion]The results indicated that TMV and PVY were the dominant origin of tobacco virus in Guizhou.%【目的】对贵州省36个县市的1547份疑似病毒病症状的烟叶样品进行检测，明确我省烟区主要病毒种类。【方法】采用双抗体夹心酶联免疫吸附法（DAS-ELISA）法。【结果】检测出1107个阳性样品，包括烟草普通花叶病毒（tobacco mosaic virus，TMV）、马铃薯 Y 病毒（potato virus Y，PVY）、黄瓜花叶病毒（cucumber mosaic virus， CMV）、烟草蚀纹病毒（tobacco etch virus，TEV）、烟草脉带花叶病毒（tobacco vein banding mosaic virus，TVBMV）等5种病毒，其中TMV和PVY的阳性检出率较高，分别为41．63％和11．70％；并以单一病毒侵染为主，另有4种类型的复合侵染。【结论】通过研究可知TMV和PVY为贵州烟草病毒病的优势毒原种类。
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