Objective] To investigate the diapause prevention mechanism of silkworm ( Bombyx mori ) , and compare a series of changes of eggshell surface after diapause prevention by HCl or DMSO treatment .[Method]The eggs were treated with HCl or DMSO at 20 and 12 h after oviposition respectively .Scan-ning electron microscope ( SEM ) was used to observe the changes of eggshell surface and aeropyle at different time points after treatment .[Result]Both HCl and DMSO treatments successfully prevented the occurrence of egg diapause , and the average hatching rates were 93.37% and 87.81% respectively . SEM observation showed clear etching effect and “lipid” shed on eggshell surface , and the aeropyle diameter increased gradually under both treatments .The above observations were more obvious for the HCl treatment compared with the DMSO treatment .[Conclusion]Eggshell surface changes may be related to the diapause prevention of silkworm .%【目的】探讨桑蚕Bombyx mori滞育阻断机理，比较HCl、二甲基亚砜（ DMSO）法阻断蚕卵滞育后卵壳表面发生的一系列变化。【方法】用HCl和DMSO分别在产后20和12 h处理蚕卵，利用扫描电镜观察卵壳表面及气孔在处理后不同时间点的变化。【结果】HCl 和DMSO 处理均能成功地阻断蚕卵滞育的发生，蚕卵平均孵化率分别为93.37％和87.81％。扫描电镜观察HCl和DMSO处理的卵壳表面蚀刻效果明显，均出现了“脂质”脱落物；并且处理后蚕卵的气孔有逐步增大的趋势，与DMSO处理相比较，HCl处理获得的现象更为明显。【结论】卵壳表面变化可能与桑蚕滞育的阻断有关。
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