[Objective] To investigate the effects of azadirachtin on cell proliferation,glucose uptake and insulin secretion in min6 cells and their possible mechanisms. [Method] The min6 cells were cultured in vitro and treated with various concentrations of azadirachtin for 48 h.Cell proliferation was measured.In the presence of 5.5 and 25.0 mmol·L-1 glucose,glucose uptake was measured and insulin secretion in min6 cells were detected by the insulin ELISA kit.[Result] Azadirachtin promoted the proliferation of min6 cells in the presence of 5.5 and 25.0 mmol·L-1 glucose compared to the control group.Compared with the 25.0 mmol·L-1 glucose condition,azadirachtin in the presence of 5.5 mmol·L-1 glucose more significantly promoted glucose uptake and insulin secretion in min6 cells.[Conclusion]Azadirachtin could affect insulin secretion by regulating glucose concentration.%[目的]研究印楝素对min6细胞增殖、葡萄糖摄取及胰岛素分泌的影响,并探讨其作用机制.[方法]不同浓度印楝素处理min6细胞48 h,检测细胞增殖量.在5.5和25.0 mmol·L-1葡萄糖条件下,检测印楝素处理细胞的葡萄糖摄取水平,胰岛素ELISA检测试剂盒检测细胞胰岛素的分泌.[结果]与对照组相比,在5.5和25.0 mmol·L-1葡萄糖条件下,印楝素均表现促进细胞增殖活性.印楝素处理下,与25.0 mmol·L-1葡萄糖条件相比,5.5 mmol·L-1葡萄糖表现出更显著地促进葡萄糖摄取和胰岛素分泌的作用.[结论]印楝素可能通过调控葡萄糖水平影响胰岛素的分泌.
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