为研究山梨酸对小鼠骨骼肌成肌细胞(C2C12)增殖、IGF-1分泌、GH/IGF系统和糖脂代谢相关基因表达的影响,试验通过培养基中添加不同浓度的山梨酸(0、0.1、1.0、10.0和100.0 μmol/L)处理C2C12细胞,采用MTT法检测细胞增殖,RIA法检测细胞IGF-1分泌量,qPCR法检测相关基因mRNA的表达水平.结果表明:1)10.0μmol/L山梨酸显著促进体积分数为10％胎牛血清(1和2d)和无血清(4和5d)培养的C2C12细胞增殖(P＜0.05)；2)0.1、1.0和100.0μmol/L山梨酸均显著抑制无血清培养的C2C12细胞IGF-1的分泌(P＜0.05)；3)1.0 μmol/L山梨酸显著上调无血清培养的C2C12细胞IGF-1R、GHR和CPT1b的mRNA表达(P＜0.05),对IGFBP3(P=0.09)、PDK4(P=0.10)和PGC1α(P =0.10)的mRNA表达量也有提高趋势.提示山梨酸可促进C2C12细胞的增殖和GH/IGF系统及糖脂代谢相关基因mRNA表达,但对IGF-1的分泌具有抑制作用.%The purpose of this experiment was to explore the effects of sorbic acid ( SA ) on proliferation, IGF-1 secretion, GH/IGF system and glucose and lipid metabolism-related genes mRNA expression in C2C12 cells. C2C12 cells were exposed to different doses of SA(0, 0. 1, 1.0, 10. 0 and 100. 0 μmol/L)to measure cell proliferation by MIT assay, IGF-1 secretion by RIA assay and transcript levels by qPCR assay. Results indicated that; 1) SA(10. 0 μmol/L) significantly promoted(P <0. 05) proliferation of cell cultured with 10% fetal bovine serum(1 and 2 d) or without serum(4 and 5 d) . 2) IGF-1 secretion was significantly suppressed (P < 0. 05 ) by 0. 1, 1. 0 or 100.0 μnol/L SA in serum-free cultured C2C12 cells. 3) Expressions of IGF-1 R, GHR and CPT1 b mRNA were significantly up-regulated in serum-free cultured C2C12 cells treated with 1. 0 μmol/L SA. The increasing trend of IGFBP3 (P =0. 09) , PDK4 (P=0. 10) and PGCla (P = 0. 10) transcript levels were also observed in serum-free cultured C2C12 cells by 1. 0 μmol/L SA treatment. This suggested that SA could increase proliferation and GH/IGF system and glucose and lipid metabolism-related genes mRNA expression but depress IGF-1 secretion in C2C12 cells.
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