This study established an identification procedure for the avianinfectious bronchitis virus, Mab-ELISA, with two strains of IBV monoclonal antibody made by an institution in China. The procedure was: coating IBV Mab (murine)-test sample (virus)-IBV positive serum from chicken-anti-chicken IgG from rabbit (AGP titres 1∶32)-sheep anti-rabbit IgG-HPR. The check using 46 IBV isolates from China with NDV, AIV-H9N2 and PPMV-I suggested that this IBV Mab-ELISA method had relatively good specificity and sensitivity.%针对禽传染性支气管炎病毒(IBV)血清型众多、临床检测不易的难题,用我国自己研制的2株IBV单克隆抗体,通过多种方法的比较与鉴定,建立起适用于定性检测IBV的包被鼠源IBV单抗的Mab-ELISA方法,其操作程序是:包被鼠源IBV单抗-被检病毒-鸡抗IBV高免血清-兔抗鸡IgG(AGP效价1∶32)-羊抗兔IgG-HPR;用本方法对国内46个IBV分离株及NDV、AIV-H9N2、PPMV-I等的检测,表明其具有较好的特异性和敏感性.
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