Objective:To investigated the effect of extractive of pericarpium trichosanthis (EPT) in atherosclerosis induced by homocysteine in rats.Methods:60 Wistar rats were randomly divided into three groups:control group,homocysteine model group,EPT experimental group,and 20 rats in each group.And 1％ methionine diet was fed to rats to induce atherosclerosis model.The serum TC,TG,LDL and Hcy concentration were detected by instrument.The change of aortic plaque was observed from aorta tissue pathological slices.The effect on the proliferation of vascular smooth muscle cells after treatment of homocysteine and EPT was detected by MTT.The expression of c-myc and c-fos gene in vascular smooth muscle cells after treatment of homocysteine and EPT was detected by RT-PCR.Results:The serum concentrations of TC,TG,LDL and Hcy in the model group were significantly higher than those in the experimental group and the control group (P ＜ 0.05),and there was no significant difference between the experimental group and the control group (P ＞ 0.05).The result of aortic tissue sections showed that the atherosclerotic plaque in the experimental group improved significantly.MTT cell proliferation detection showed that after high homocysteine treatment of vascular smooth muscle cells,the cell proliferation rate was significantly higher than that of the control group and the experimental group (P ＜ 0.05),while the experimental group and the control group had no statistical difference (P ＞ 0.05).After high homocysteine treatment of vascular smooth cells,the expression of c-myc and c-fos in the cells was significantly higher than that in the control group and the experimental group (P ＜ 0.05),but there was no significant difference between the experimental group and the control group (P ＞ 0.05).Conclusion:EPT can inhibit atherosclerosis induced by homocysteine.%目的:探讨在大鼠中瓜蒌皮提取物如何影响同型半胱氨酸诱导的动脉粥样硬化.方法:将60只Wistar大鼠随机分为3组:对照组20只、同型半胱氨酸模型组20只、瓜蒌皮提取物实验组20只,采取1％蛋氨酸喂养诱导动脉粥样硬化模型.检测血清中TC、TG、LDL、Hcy浓度,主动脉组织病理切片观察斑块情况,MTT试剂检测同型半胱氨酸、瓜蒌皮提取物处理后对血管平滑肌细胞增殖影响,RT-PCR检测同型半胱氨酸、瓜蒌皮提取物处理后血管平滑肌细胞内c-myc、c-fos基因的表达情况.结果:同型半胱氨酸模型组血清中TC、TG、LDL、Hcy浓度显著高于实验组和对照组(P＜0.05),实验组和对照组差异无统计学意义(P＞0.05).主动脉组织切片显示实验组动脉粥样硬化斑块得到显著改善.MTT细胞增殖检测发现,高同型半胱氨酸处理血管平滑肌细胞后,细胞增殖速度显著高于对照组和实验组(P＜0.05),而实验组和对照组间差异无统计学意义(P＞0.05).高同型半胱氨酸处理血管平滑细胞后,细胞内c-myc、c-fos基因表达显著高于对照组和实验组(P＜0.05),而实验组和对照组间差异无统计学意义(P＞0.05).结论:瓜蒌皮提取物可以抑制同型半胱氨酸诱导的动脉粥样硬化.
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