目的 观察槲皮素对成年大鼠局灶性脑缺血后侧脑室室管膜下区(SVZ)细胞增殖的影响.方法 线栓法制作大鼠右侧大脑中动脉阻塞模型,术后6 h腹腔注射槲皮素(50mg/kg,1次/3 d),术后4 h腹腔注射BrdU(50mg/kg,1次/d),分别于缺m第7、14、21天采用免疫荧光染色观察侧脑室SVZ BrdU阳性细胞数.结果 脑缺血第7天,缺血侧SVZ BrdU阳性细胞较假手术组明显增多,第14天达峰值,第21大减少(P<0.01).槲皮素组第7天时,缺血侧SVZ BrdU阳性细胞亦明显增多,并随着缺血时间延长明显增加;与缺血组比较,槲皮素组7、14和21 d缺血侧SVZ BrdU阳性细胞数均显著增加(P<0.01),至21 d仍保持高水平.结论 槲皮素可维持成年大鼠局灶性脑缺血后侧脑室SVZ的细胞增殖在较高水平.%Objective To study the effect of quercetin on the proliferation of neural stem cells in the subventricular zone (SVZ) of rats after focal cerebral ischemia. Methods An adult rat model of middle cerebral artery occlusion (MCAO) model was established by placement of an intraluminal filament at the origin of the MCA. Quercetin was administered intraperitoneally in the rats at a dose of 50 mg/kg every 3 days starting at 6 h after MCAO, and BrdU (50 mg/kg daily) was also injected intraperitoneally starting at 4 h after MCAO. BrdU-positive cells in the SVZ were counted at 7, 14 and 21 days after MCAO. Results Compared with the sham-operated group, the rats in the ischemic model group showed significantly increased BrdU-positive cells in the ipsilateral SVZ 7 days after MCAO, reaching the peak level on day 14 and beginning to decrease on day 21 (P<0.05). The number of ipsilateral BrdU-positive cells in quercetin group was significantly greater than that in the model group on days 7,14 and 21 (P<0.05), and maintained the high level on day 21. Conclusion Quercetin can maintain a high level of neural stem cell proliferation in the SVZ after focal cerebral ischemia in adult rats.
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