Eukaryotic translation initiation factor 4e (eIF4E) is a necessary factor for many viruses infecting plants.The plant expression vectors of Cp-eIF4E and Cp-eIFiso4E were constructed,fused with green fluorescent protein (GFP),and transformed into the protoplasts of tobacco leaf to study the subcellular localization of papaya eIF4E family.The results showed that Cp-eIF4E and Cp-eIFiso4E were expressed in the cytoplasm and nucleus.This study would lay a foundation for further study of the interactions between the papaya eIF4E family proteins and viruses,and their functions in virus infection.%真核翻译起始因子eIF4E是多种病毒侵染植物的必须因子.笔者通过扩增番木瓜eIF4E家族基因Cp-eIF4E和Cp-eIFiso4E,分别构建他们与绿色荧光蛋白(GFP)融合的植物表达载体,转化烟草叶片的原生质体后研究了番木瓜eIF4E家族蛋白的亚细胞定位情况.结果表明,Cp-eIF4E和Cp-eIFiso4E在细胞质和细胞核中均有表达.本研究为进一步探讨番木瓜eIF4E家族蛋白与病毒的互作机制以及解析其在病毒侵染中的功能奠定了理论基础.
展开▼
