A method for determination ginsedoside Re and Rh2 has been developed by RP-HPLC. Agilent Ex-tend C18(4.6 mm × 150 mm,5 µm)served as the stationary phase. The mobile phase consists of actonitrile and 0.05% acetic acid in term of gradient operation. Detection wavelength was 203 nm,column temperature 30.0℃,flow rate,1.00 mL/min. The analytical linearity range of ginsenoside Re and Rh2 were 0.17~456.6 mg/L and 6.00~449.4 mg/L,respectively .The recoveries of ginsenoside Re and Rh2 were 99.60% and 99.78%,respectively. The method developed is rapid,simple and accurate and can be used to determine the ginsenoside Re and Rh2 of Radix et Rhizoma Ginseng and Panax quinquefolium L extraction product.%利用反相-高效液相色谱法(RP-HPLC)建立了快速测定人参皂苷Re和Rh2含量方法。色谱柱为Agilent Extend C18（4.6×150 mm，5µm），流动相为乙腈-0.05%乙酸梯度洗脱溶液，流速1.00 mL/min，波长203 nm，柱温30.0℃。人参皂苷Re和Rh2的线性范围分别为0.17~456.6 mg/L和6.00~449.4 mg/L；人参皂苷Re和人参皂苷Rh2的平均加标回收率分别为99.60%和99.78%。本方法快速、灵敏、准确性好，可用于测定人参和西洋参提取物中人参皂苷Re和人参皂苷Rh2的含量。
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