以优良无性系中山杉301茎段为外植体,通过在MS或1/2MS培养基中添加不同浓度和比例的NAA、6-BA、IBA、KT对其进行芽诱导分化、 增殖培养和生根诱导,以提升中山杉微体快繁技术研究水平.研究结果表明:以中山杉301带腋芽幼嫩枝条为外植体可以实现微体快繁.MS培养基中NAA含量较高时可以增加萌芽数量,6-BA含量过高对萌芽有抑制作用;诱导分化培养基配方以MS+NAA 0.2mg/L+6-BA 0.4mg/L效果最佳.增殖培养配方以MS+NAA 0.3mg/L+6-BA 0.4mg/L效果最佳,丛芽分化良好,增殖倍数3.8.相同浓度的IBA和NAA以1/2MS生根诱导数量远远高于MS培养基,生根诱导配方以1/2MS+IBA 0.3mg/L+NAA 0.2mg/L效果最好,较相同激素条件下的MS培养基生根率提高8%.%To explore the micro-propagation, the stems of Taxodium hybrid'zhongshanshan' 301 superior clones were applied as explants to conduct experiments of the bud differentiation, enrichment culture and root induction in MS and 1/2MS medium with different concentration and proportion of NAA, 6-BA, IBA and KT.The results showed that: The explant which was stem piece with bud of Taxodium hybrid'zhongshanshan' 301 presented well for propagation.There were more lateral buds if more NAA was added into MS medium, and the content of 6-BA was too high to inhibit germination.Optimum directions for culture media for inducement and differentiation was MS+NAA 0.2mg/L+6-BA 0.4mg/L.Optimum directions for culture media for proliferation was MS+NAA 0.3mg/L+6-BA 0.4mg/L, and bud differentiation was good, multiplication rate was 3.8.Optimum directions for culture media for root inducing was 1/2MS+IBA 0.3mg/L+NAA 0.2mg/L, and the number of IBA and NAA on rooting was significantly higher in 1/2MS medium than that in MS medium, rooting rate increased by 8%compared to the same hormone under the condition of MS culture, significant difference.
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