目的 研究细菌脂多糖(LPS)直接促进破骨细胞分化的调节作用.方法 建立RANKL和M-CSF诱导骨髓单核细胞分化为成熟破骨细胞的体外培养方法；采用抗酒石酸酸性磷酸酶染色(TRAP)观察LPS对RANKL预处理的破骨细胞前体分化调节作用；RT-PCR检测LPS对RANKL预处理的破骨细胞前体表达TNF-α、IL-1β.结果 LPS通过刺激破骨前体细胞表达和分泌TNF-α、IL-1β促进破骨细胞向成熟分化,并且不依赖于RANKL-RANK途径.结论 LPS促进RANKL预处理的破骨细胞前体向成熟分化的途径可能是通过增加其自分泌TNF-α和IL-1β完成的.%Objective To study the stimulation of osteoclast differentiation by lipopolysaccharide (LPS). Methods Osteoclasts were prepared in the presence of receptor activator of nuclear factor-κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) from bone marrow mononuclear cells; tartrate-resistant acid phosphatase (TRAP) staining was used to observe osteoclastogenic activity induced by lipopolysaccharide (LPS); RT-PCR was applied to detecte tumor necrosis factor-α (TNF-α) and interleukin 1(3 (IL-lβ) expressions in RANKL-pretreated precursor osteoclasts. Results LPS could induce osteoclast differentiation in RANKL-pretreated precursor osteoclasts and was independent of RANKL-RANK pathway. LPS induced the expression of TNF-α and IL-1β in osteoclast precursors no matter whether they were pretreated with RANKL or not, and these cytokines mediated LPS effect in an autocrine mechanism. Conclusion LPS stimulates osteoclastogenesis in RANKL-pretreated cells by increasing the secretion of TNF-α and IL-1β.
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