目的：探讨维药异常黑胆质成熟剂对异常黑胆质型支气管哮喘大鼠尿液代谢物变化的影响。方法应用多因素复合作用建立异常黑胆质证载体动物模型，采用卵清蛋白（OVA）致敏和雾化激发方法建立异常黑胆质型支气管哮喘病证模型，将SD大鼠（84只）随机分为空白对照组、异常黑胆质证载体模型组、异常黑胆质证型支气管哮喘模型组、异常黑胆质成熟剂给药组（分为大、中、小剂量组）、地塞米松给药阳性对照组。收集各组大鼠尿液，利用核磁共振波谱（1 H －NMR）技术测定核磁共振氢谱，建立大鼠尿液的代谢指纹谱，运用正交偏最小二乘判别分析（OPLS －DA）法分析各组大鼠尿液中代谢产物差异。结果与空白对照组相比，异常黑胆质型哮喘模型组柠檬酸、肌酸酐、甘氨酸等重要氨基酸含量降低，差异有统计学意义（P ＜0．05）；灌胃异常黑胆质成熟剂之后，异常黑胆质型哮喘模型组降低的肌酸酐及乙酸等含量明显增高，牛磺酸、甜菜碱等含量降低，与异常黑胆质型哮喘模型组比较差异有统计学意义（P ＜0．05）；与异常黑胆质型哮喘模型组相比，地塞米松给药阳性对照组马尿酸、乳酸、丙氨酸等含量升高（P ＜0．05）。结论异常黑胆质成熟剂对异常黑胆质型哮喘大鼠代谢具有显著影响，其作用机制可能是通过作用于异常黑胆质型支气管哮喘大鼠体内氨基酸代谢与能量代谢途径而发挥预防及治疗异常黑胆质型支气管哮喘的作用。%Objective To study the effect of Abnormal Savda Munziq on urine metabolic changes of Abnor‐mal Savda Syndrome carrying asthma rat model .Methods Multiple factors was used to establish Abnor‐mal Savda rat model ,according to the Traditional Uyghur Medicine .By sensitization and challenge with ovalbumin (OVA) Abnormal Savda Syndrome carrying rat model was established further .84 SD rats were randomly divided into black control group ,Abnormal Savda Syndrome control group ,Abnormal Savda Syndrome carrying asthma control group ,Munziq group (respectively ,low dose ,middle dose and high dose group) and Dexamethasone (DXM ) group .Nuclear Magnetic Resonance Spectroscopy (1 H‐NMR) technique was used for determination of collected urine ,metabolic fingerprints of rat urine was estab‐lished ,and orthogonal to partial least squares discriminant analysis (OPLS‐DA) method was used for the analysis of differences among metabolites in urine from all goups .Results Various kinds of amino acids from Abnormal Savda Syndrome carrying asthma control group are markedly lower ,including citric acid , glycine ,creatinine ,compared to black control group ,and the differences were statistically significant (P<0 .05);Afrer oral administration of Abnormal Savda M unziq ,creatinine ,acetic acid in Abnormal Savda Syndrome carrying asthma rat urine increased ,and taurine ,betaine were reduced ,compared with Abnor‐mal Savda Syndrome carrying asthma control group ,the differences were statistically significant (P <0 .05) .Compared with Abnormal Savda Syndrome carrying asthma control group ,hippuric acid ,lactic acid , alanine were higher in dexamethasone group ,and the differences were statistically significant (P <0 .05) . Conclusion Abnormal Savda Munziq has significant effects on Abnormal Savda Syndrome carrying asthma rat urine metabolites ,the mechanism of w hich may achieved by readjusting the altered amino acid metabo‐lism and energy metabolism caused by Abnormal Savda Syndrome carrying asthma .
展开▼
