目的 研究老鼠簕对四氯化碳( CCl4)诱导的肝纤维化大鼠肝组织Toll样受体4(TLR4)表达的影响.方法 Wistar大鼠随机分为空白对照组、模型对照组、秋水仙碱组、老鼠簕乙醇提取物低剂量组和老鼠簕乙醇提取物高剂量组.除空白对照组外,其余4组用GCl4制备大鼠肝纤维化模型.秋水仙碱组、老鼠簕乙醇提取物低剂量组和老鼠簕乙醇提取物高剂量组分别于4周后给予相应的药物,空白对照组和模型对照组给予等容量的生理盐水,每日1次.8周后处死大鼠.逆转录-聚合酶链反应(RT-PCR)检测肝组织中TLR4 mRNA的表达.结果 RT-PCR结果显示,与空白对照组相比,模型对照组TLR4 mRNA表达增强(P＜0.01)；老鼠簕乙醇提取物干预组(1.0、2.0 g·kg-1)较模型对照组TLR4 mRNA表达减弱(P＜0.05).结论 老鼠簕能够抑制肝纤维化大鼠肝组织TLR4的表达.%Objective To explore the effect of Acanthus ilicifolius Linnseus on Toll-like receptor 4 ( TLR4 ) expressions of the hepatic fibrosis induced by carbon tetrachloride( CC14 ) in rats. Methods Wistar rats were randomly divided into normal control group, model control group, colchicine group, low dosage group and high dosage group of ethanol extract of Acanthus ilicifolius(EEA). Except the normal control group,the other four groups were injected CC14 to establish hepatic fibrosis model. Four weeks later, the colchicine group, the EEA low dosage group and the EEA high dosage group were treated with colchicine and EEA respectively, the normal control group and the model control group were treated with normal saline. All rats were sacrificed administration for eight weeks. The TLR4 mRNA expressions were detected by reverse transcription polymerase chain reaction. Results Compared with normal control group,the levels of TLR4 expression in model control group were elevated(P < 0. 01) ;the levels of TLR4 expression in the EEA-treated( 1. 0,2. 0 g·kg-1 ) groups were decreased compare with model control group(P <0. 05). Conclusion Acanthus ilicifolius Linnseus can inhibit TLR4 expressions in liver fibrotic rats.
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