Bonetissue engineering scaffold material and its preparation is very important to its performance.Compared with the single polyvinyl alcohol (PVA) gel,silk fibroin (SF)-polyvinyl alcohol (PVA) composite gel not only increases the stress but also improves the toughness of the scaffold.In this study,silk fibroin (SF)-polyvinyl alcohol (PVA) composite gel can be prepared by mixing silk fibroin (SF) powder with polyvinyl alcohol (PVA) crystal in 1 ∶ 4 by utilizing water bath heating method.The silk fibroin (SF)-polyvinyl alcohol(PVA) composite gel is prepared by mixing with hydroxyapatite (HA) based on the low temperature freeze drying technology.The test sample is prepared,which the compressive strength is 31.2 MPa and the elastic modulus is 11.07 MPa.The maximum total deformation is lower than that of single PVA/HA samples,and it is not easy to break and crush.The experimental results show that the pore diameter of the HA/SF-PVA composite material is 15～350 μm,and the pore is equally distributed and the connectivity is good.The 3D printing composite scaffold is well formed and the pore is obvious without the expansion of silk.When the silk fibroin polyvinyl alcohol (1 ∶ 4) composite gel was heated by water bath,it is mixed with HA according to the ratio of 1.5 ∶ l.The mechanical properties and pore size of HA/SF-PVA composites prepared by utilizing this method are benefit to the repair of bone defects and the growth of bone cells.%组织工程骨支架材料及制备对支架性能影响至关重要.同单一聚乙烯醇凝胶相比,丝素蛋白-聚乙烯醇复合凝胶不仅可以增加支架所承受应力而且可以提高支架韧性.研究证明,使用水浴加热方法,将丝素蛋白粉末与聚乙烯醇晶粒按照1∶4混合加热,制成丝素蛋白-聚乙烯醇复合凝胶.再与羟基磷灰石充分混合,基于低温冷冻干燥技术,制备成试验试样.压缩试验测量最大压缩强度为31.2 MPa,弹性模量为11.07 MPa,最大总变形低于单一聚乙烯醇/羟基磷灰石试样,且压缩时不易发生断裂及粉碎,综合力学性能较好.电镜实验观察得到羟基磷灰石/丝素蛋白-聚乙烯醇复合材料试样孔径为15 ～ 350 μm,微孔分布均匀且连通性较好.水浴加热后的丝素蛋白-聚乙烯醇(1∶4)复合凝胶与羟基磷灰石按照1.5∶1混合时,3D打印复合材料支架成形好,无丝材膨胀,孔隙明显.使用此方法制备的羟基磷灰石/丝素蛋白-聚乙烯醇复合材料,力学性能及孔径大小利于负重部位骨缺损修复及骨细胞的生长.
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