• 主题
高级搜索  >
历史搜索 清空历史
首页> 中文期刊> 《浙江大学学报（英文版）（A辑：应用物理和工程）》 >Effect of temperature on batch elastase production by Bacillus sp. EL31410

Effect of temperature on batch elastase production by Bacillus sp. EL31410

         
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

＜正＞The production of elastase by Bacillus sp. EL31410 at various temperatures was investigated. In order to study the effect of temperature on elastase fermentation, different cultivation temperatures, ranging from 39 ℃ to 28 ℃, were evaluated in shake flask. The result indicated that 37 ℃ was best for cell growth at earlier stage; while maximum elastase activity was obtained when the cells were cultivated at 30 ℃. This result was verified by batch fermentation in 5-L bio-reactor under 37 ℃ and 30 ℃ temperature, respectively. The specific cell growth rate at 37 ℃ was higher than that at 30 ℃ during earlier stage of cultivation. The maximum value [5.5 U/（h-g DCW）] of elastase formation rate occurred at 24 h at 30 ℃ compared to 4.6 U/（h-g DCW） at 30 h at 37 ℃. Based on these results, two-stage temperature shift strategy and oscillatory temperature cultivation mode were evaluated in the next study. When compared to single temperature of 37 ℃ or 30 ℃, both two-stage temperature shift strategy and os

著录项

相似文献

  • 中文文献
  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号