目的 通过对本所长期室内人工饲育的不同季节不同龄期的棉铃虫(Heliothis armigera)进行体内外的微生物分离培养和鉴定,掌握棉铃虫体内外微生物携带情况.方法 利用普通培养基和真菌培养基分别对棉铃虫体内外进行微生物分离培养、纯化,通过菌落形态观察、革兰氏染色和显微镜观察进行初步鉴定,然后应用16SrRNA和18SrDNA分析技术对分离的细菌和真菌进行进一步的鉴定.同时,利用PCR技术对棉铃虫的高度致病病毒-核型多角体病毒(HaNPV)进行扩增检测.结果 分离培养得到4株芽孢杆菌(Bacillus):分别为枯草芽孢杆菌(Bacillus subtilis Cohn),地衣芽孢杆菌(Bacillus licheniformis),蜡状芽孢杆菌(Bacillus cereus)和芽孢杆菌(Bacillus).4株真菌(fungi):其中2株为酵母菌(Yeasts),1株为青霉菌(Aenicil lium Link),1株为曲霉菌(Aspergillus Micheli).检测核型多角体病毒(HaNPV)为阴性.结论 室内人工饲养的棉铃虫生长状态良好,虫体健康,未携带致病微生物,为科研提供的实验昆虫质量稳定可靠.%Objective Microbial isolation, culture, and identification in vitro and in vivo were carried out on Heliothis armigera of different seasons and different ages that were bred indoor in the institute to investigate the carriage of microorganisms. Methods Common culture medium and fungi culture medium were used for the in vivo and in vitro microbial isolation, culture and purification of heliothis armigera, respectively. The preliminary identification was performed through colony morphological observation, Gram's staining and microscopic observation. Then 16SrRNA and 18SrRNA analysis technology was applied for further identification of isolated bacteria and fungi. Meanwhile, PCR was used for amplification detection of highly pathogenic HaNPV for Heliothis armigera. Results Four strains of Bacillus were isolated, which belonged to Bacillus subtilis Cohn, Bacillus licheniformis, Bacillus cereus and Bacillus, respectively. Four strains of fungi were isolated, including 2 strains of Yeasts, one strain of Aenicillium Link and one strain of Aspergillys Micheli. The result of HaNPV detection was negative.
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