目的 串联合成犬瘟热病毒N蛋白抗原表位基因,并构建其杆状病毒表达载体.方法 利用生物信息学软件,预测N蛋白的抗原表位,串联合成这些抗原表位.利用定向克隆的方法,将该基因片段连接到杆状病毒表达载体pFastBac-Hta.结果 获得了包含犬瘟热病毒N蛋白抗原表位的基因,并成功构建pFastBac-N合成表达载体.结论 该实验为犬瘟热病毒N蛋白抗原表位基因在杆状病毒内的表达奠定了基础.%To synthesize the gene including the CDV-N protein antigen'epitopes, and construct the baeulovirus expression vector. Methods The N protein antigen epitopes was predicted by bioinformatics software and synthesized tandem. The fragment was cloned into the baeulovirus expression vector pFastBac-Hta. Results The gene including the CDV-N protein antigen epitopes was obtained and successfully constructed the pFastBac-Nsynthesis expression vector. Conclusion The experiment laid the foundation for the CDV-N protein antigen epitopes expression in baeulovirus.
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