目的 对分离纯化得到的制黄精多糖PS P-1-A进行体外抗氧化活性测试.方法 制黄精经分离纯化后得到制黄精粗多糖(PSP)及PSP-1-A,采用体外抗氧化实验法测定PSP及PSP-1-A清除2,2-二苯基-1-苦肼基(DPPH)自由基、对羟基自由基的能力及总抗氧化能力.结果 测得PSP及PSP-1-A在体外均能明显清除DPPH自由基、对羟基自由基和ABTS+自由基,且其体外抗氧化功能在一定范围内随浓度的增大而增大.结论 PS P及PS P-1-A在一定浓度下均具有明显的体外抗氧化作用.%Objective To research the in vitro antioxidative effect of separated and purified polysaccharide PSP-1-A from Polyg-onatum Sibiricum Redoute .Methods The polysacchrade PSP-1-A was obtained from Polygonatum Sibiricum Redoute by separation and purification .The in vitro antioxidative experimental method was adopted to determine the ability of PSP and PSP-1-A for clear-ing DPPH free radical and hydroxyl free radical ,and total antioxidant capability .Results It was detected that PSP and PSP-1-A could obviously clear in vitro DPPH free radical ,hydroxyl radical and ABTS+ radical ,moreover its in vitro anti-oxidative effect was increased with its concentration increase in some range .Conclusion PSP and PSP-1-A have significant antioxidative activity in vitro under a certain concentration .
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