Objective To investigate the distribution of drug resistance gene in patients with tuberculosis(TB) co-infected with coagulase negative Staphylococcus(CNS),so as to provide guidance to fight TB co-infections.Methods Patients in our hospital who confirmed tuberculosis complicated with infections were collected,and drug resistance of 54 strains of CNS were analyzed,and polymerase chain reaction(PCR) was used to detect the resistance genes.Results Drug susceptibility of 54 strains of CNS showed that,the resistance rate to benzene azole Westwood coagulase negative Staphylococcus(MRCNS) detection was 92.3 ％,the resistant rate of penicillin was 96.2 ％,which of erythromycin,gentamicin and tetracycline were 86.5 ％,75.0 ％ and 86.5 ％,respectively.Strain which resistant to vancomycin intermediary was also found.PCR amplification showed that the 50 strains had mecA gene,46 strains had aac(6')/aph(2') gene,26 strains had aph(3')Ⅲ gene,20 strains had ermA gene,15 strains had tetM gene,and positive rates were 92.5 ％,85.1％,48.1％,37.0 ％ and 27.8 ％,respectively.Conclusion TB associated infections of CNS has showed multidrug resistance,carrying a variety of resistance genes,and drug resistance is serious,resistance genes and resistance are closely re lated.%目的 了解结核合并感染凝固酶阴性葡萄球菌(CNS)患者的耐药基因分布情况,为治疗结核合并感染患者提供指导.方法 收集该院已确诊肺结核合并感染的患者,对分离的54株CNS进行耐药性分析,并采用PCR方法检测其耐药基因.结果 54株CNS的药敏结果显示,耐苯唑西林凝固酶阴性葡萄球菌(MRCNS)检出率为92.3％,青霉素耐药率达96.2％,红霉素、庆大霉素和四环素耐药率分别为86.5％、75.0％和25.9％,发现耐万古霉素中介株;采用PCR扩增法检测其耐药基因,检出mecA基因50株,aac(6')/aph(2')基因46株,aph(3')-Ⅲ基因26株,ermA基因20株,tetM基因15株,阳性率分别为92.5％、85.1％、48.1％、37.0％、27.8％.结论 结核合并感染CNS呈多重耐药性,携带多种耐药基因,耐药性严重,临床应给予重视.
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