Objective To assess the association of human apiloma virus(HPV) infection with esophageal squa‐mous cell carcinoma(EC) ,and elucidate the putative role of HPV infection in the etiology of esophageal carcinoma in mianyang area .Methods A total of 166 esophageal tissue samples were collected and extracted DNA ,and 114 sam‐ples were esophageal cancer samples in study group ,52 samples were benign esophageal diseases biopsy specimens in control group .Surface Plasmon Resonance(SPR)technique was applied to detect HPV genotypes .Results A total of 46 case(40 .4% )were HPV positive among 114 EC tissues ,while 3 case(5 .8% )were HPV positive among 52 con‐trols .The most positive percentage of two subtypes infection of HPV was HPV16 and HPV18 ,followed by HPV58、6、31、33、35、51、11 .Ten cases were infected with two or more than two subtypes .Conclusion The high rate of HPV in the esophageal carcinoma samples suggested that HPV infection might be an important etiologic factor in the de‐velopment of esophageal cancer in Mianyang area .%目的：了解绵阳地区食管鳞癌感染与人乳头瘤病毒（HPV）的关系，探讨HPV在食管癌病因学中的作用。方法收集食管组织标本166例（试验组为食管癌患者活检标本114例，对照组为食管良性疾病活检标本52例），提取组织标本DNA，采用表面等离子谐振法，检测组织标本HPV感染情况及感染HPV基因型别。结果114例食管癌组HPV检出率为40．4％，对照组HPV检出率为5．8％，HPV感染基因型从高到底依次为16、18、58、6、31、33、35、51、11型，食管鳞癌感染HPV主要的基因型为16（23／114）、18（10／114）型，在食管鳞癌HPV阳性病例中混合感染10例占21．7％，16型混合感染7例占15．2％（7／46），18型混合感染3例占6．52％。结论绵阳地区食管癌活检组织中有HPV存在，HPV感染可能和绵阳地区食管癌的发生存在相关，可能是本地区食管鳞癌发病的危险因素之一。
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