Objective To establish a reference measurement procedure for the determination of serum electrolytes(potassium,sodium,calcium and magnesium) based on ion chromatography,and to evaluate its analytical performance. Methods Samples were prepared by a simple digestion and determined by ion chromatography. Fresh sample from patients and the International Federation of Clinical Chemistry and Laboratory Medicine External Quality Assessment Scheme for Reference Laboratories in Laboratory Medicine(RELA) sample were used. The accuracy,imprecision and methodology comparison with inductively coupled plasma mass spectrometry(ICP-MS) were evaluated. Results A total of 1.0 mL of serum sample was digested with 2 mL nitric acid. The samples were redissolved and determined by ion chromatography under the optimal eluent concentration of 32 mmol/L methane-sulfonic acid. The accuracy and imprecision were <1.0%. The results were comparable with the reference values obtained by ICP-MS. Conclusions The established reference measurement procedure for the determination of serum potassium,sodium,calcium and magnesium has the advantages of simple,low-cost,high accuracy and good stability compared to ICP-MS. It may provide an effective way for traceability.%目的 建立一种基于离子色谱技术的血清电解质(钾、钠、钙、镁)候选参考测量程序并评价其性能.方法 采用一种简单的湿法消化方法 对血清样本进行前处理,然后采用离子色谱仪检测.以患者新鲜血清及国际临床化学和检验医学联合会参考实验室外部质量评价计划(RELA)样本作为检测样本,对候选参考测量程序的正确度和不精密度进行评估,同时与决定性方法 [电感耦合等离子体质谱法(ICP-MS)]比较.结果 2 mL硝酸可将1.0 mL血清样本中的蛋白完全去除.32 mmol/L甲基磺酸淋洗液能将钾、钠、钙、镁实现基线分离.建立的候选参考测量程序的正确度和不精密度均在1.0%以内,与ICP-MS具有良好的可比性.结论 成功建立了基于离子色谱技术的检测血清钾、钠、钙、镁的候选参考测量程序,相对于ICP-MS,具有操作简便、运行成本低、正确度高、稳定性好等优点,在临床检验的量值溯源中更具实用价值.
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