Objective To evaluate B-type natriuretic peptide quantification by single-epitope sandwich ( SES) combined with cyclic enhanced immunofluorescent assay(CEIFA).Methods By SES combined with CEIFA, BNP was determined.The precision, linear range, function sensitivity, recovery rate and sample stability were evaluated, and 45 samples were determined by the method, which the results were evaluated with those of SIEMENS ADVIA Centaur CP chemiluminescence analyzer (Centaur CP).Results The within-run and between-run coefficients of variation (CV) were 4.38%-7.84% and 10.47%-11.21%, respectively.The linear range was 25-5 000 pg/mL.The function sensitivity was 12.5 pg/mL.The recovery rate was 95.04%-109.2%.The correlation formula between the novel method and Centaur CP was Y =0.998 3X -22.38 (r =0.969, P <0.01).The request of sample stability of SES combined with CEIFA was lower than that of Centaur CP.Conclusions SES combined with CEIFA for BNP quantification has a good performance, and there are some advantages compared with traditional double-antibody sandwich chemiluminescence assay.%目的：初步评价单一位点夹心抗体（SES）法结合循环增强荧光免疫技术（CEIFA）检测 B 型钠尿肽（BNP）的性能。方法用 SES 法结合 CEIFA 仪检测 BNP，对其精密度、线性范围、功能敏感性、回收率、样本稳定性及与西门子 ADVIA Centaur CP 化学发光仪（简称 Centaur CP）的相关性进行评估。结果 SES 法结合 CEIFA的批内和批间变异系数（CV）分别为4．38％～7．84％和10．47％～11．21％。线性范围为25～5000 pg／mL。功能敏感性为12．5 pg／mL。回收率为95．04％～109．2％。与 Centaur CP 比较，其回归方程为 Y ＝0．9983X －22．38（r ＝0．969，P ＜0．01）。对样本稳定性的要求低于 Centaur CP。结论 SES 法结合 CEIFA 测定 BNP 具有较好的性能，与传统的双抗体夹心发光法比较有一定优势，适合临床急诊实验室使用。
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