目的：研究中药五味子对头孢哌酮-舒巴坦耐药和非耐药的鲍曼不动杆菌的杀菌和抑菌作用。方法制备五味子浸液，采用等倍稀释法进行体外抑菌和杀菌试验，分别测定五味子药液对头孢哌酮-舒巴坦耐药和非耐药的鲍曼不动杆菌最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。结果五味子对头孢哌酮-舒巴坦耐药和非耐药的鲍曼不动杆菌的MIC50分别为15．6和7．8 mg/mL，在此药物浓度处，对头孢哌酮-舒巴坦非耐药的鲍曼不动杆菌的抑菌率均高于耐药的菌株（χ2＝4．26、3．98，P均＜0．05）；当药物浓度达到31．3 mg/mL时对二者的抑菌率均为100％；五味子对头孢哌酮-舒巴坦耐药和非耐药的鲍曼不动杆菌的MBC50均为31．3 mg/mL，杀菌率差异无统计学意义（χ2＝0．74，P＞0．05）。结论五味子在体外对头孢哌酮-舒巴坦耐药和非耐药的鲍曼不动杆菌均有一定的杀菌和抑菌作用，对头孢哌酮-舒巴坦耐药的菌株MIC略高，可尝试用于临床鲍曼不动杆菌感染的治疗。%Objective To observe the bactericidal and bacteriostatic effects of Schisandra chinensis fruit against Acinetobacter baumannii which was resistant to cefoperazone-sulbactam or non-resistant to cefoperazone-sulbactam. Methods The extract of Schisandra chinensis fruit was prepared,bacteriostatic and bactericidal test in vitro by tube double dilution method was performed to observe their minimal inhibitory concentration (MIC)and minimal bactericidal concentration (MBC ) to Acinetobacter baumannii. Results The MIC50 of Schisandra chinensis fruit against Acinetobacter baumannii resistant to cefoperazone-sulbactam or non-resistant to cefoperazone-sulbactam were 1 5 .6 and 7.8 mg/mL,and on this drug concentration,the inhibitory rate against non-resistant to cefoperazone-sulbactam Acinetobacter baumannii was higher than that against resistant to cefoperazone-sulbactam (χ2 =4.26 and 3.98,P<0.05).When the drug concentration reached to 31 .3mg/mL,the inhibitory rates were all 1 00%.The MBC50 of Schisandra chinensis fruit against Acinetobacter baumannii which was resistant to cefoperazone-sulbactam or non-resistant to cefoperazone-sulbactam were 31 .3 mg/mL,and the bactericidal rate had no statistical significance (χ2 =0.74,P>0.05).Conclusions The Schisandra chinensis fruit has certain bactericidal and bacteriostatic effects on Acinetobacter baumannii which was resistant to cefoperazone-sulbactam or non-resistant to cefoperazone-sulbactam in vitro,and the MIC against Acinetobacter baumannii is a little higher.It suggests that Schisandra chinensis fruit could be used for the clinical treatment of Acinetobacter baumannii.
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