BAI1(脑血管生成抑制因子1)因其具有抑制血管生成的作用而得名,研究表明肿瘤的发生可能与BAI1的低表达有关.为了进一步探索BAI1的作用机制,运用改良的Red重组系统和低拷贝中间载体,利用50 bp的同源重组序列直接从BAC载体中克隆长片段的小鼠基因组序列；将得到的基因组序列再次通过重组和改造,构建了BAI1基因的完全敲除并带有报告基因的打靶载体,为后续的构建BAI1基因敲除小鼠模型、在动物体内研究基因功能奠定了基础.%Brain angiogenesis inhibitors 1 (BAH) is a transmembrane protein named as its function of inhibiting the growth of new blood vessels. It has been identified that low expression of BAI1 protein may be associated with the occurrence of tumors. To find out the further function and mechanism of BAIl, a gene knockout mouse will be essential. The first step to generate a gene knockout mouse model is to construct a targeting vector for homologous recombination in mouse embryonic stem cells. Using Red-Cre system as well as Low- and intermediate-copy-number vector, 50 bp regions of homology was used to clone long regions of mouse genome from BAC strain. Then the obtained genomic sequence was modified and recombined again, and the aimed complete-knockout targeting vector of BA I] gene which carried reporter gene was constructed successfully. It has been confirmed that the BAH targeting vector is important to its continuing studies of constructing the knockout mouse model and revealing the role of BAIl gene in animals.
展开▼
