在植物内生固氮菌系统发育进化关系研究中,常用的方法有形态学与蛋白质水平法、数值分类和自动化鉴定法、化学分类法、分子遗传学方法等.本文简要介绍了常用方法的关键技术,并归纳了它们的优缺点.生物学的研究进入基因组时代后,随着高通量DNA测序技术在微生物学领域应用的迅速发展,全基因组测序被应用到微生物系统发育进化研究中,然而目前并未发现对已测全基因组序列的植物内生固氮菌进行系统总结.本文在对已测序植物内生固氮菌进行归纳的基础上,又详细研究了基于基因组数据的几种具有代表性的新方法(ANI分析法、最大唯一匹配指数法、核心基因组分析、组分矢量法、基因流动性分析),并结合目前系统发育进化研究常用方法,对植物内生固氮菌系统发育进化研究趋势进行总结和展望,旨在使植物内生固氮菌的系统发育进化关系研究在精确度、可靠性等方面有所突破.%To study phylogenetic relationships of endophytic nitrogen fixing bacteria in plants,common methods include morphology and protein level identification,numerical classification and automatic identification,chemical classification and identification,and molecular genetic identification.This paper introduces the key techniques of common methods,and discusses their advantages and disadvantages.With the development of high-throughput DNA sequencing technology in the field of microbiology,the whole genome sequencing has been applied to the study of microbial phylogenetic evolution.However,there is no systematic review of nitrogen-fixing endophytes whose genomes have been sequenced.A preliminary study on measuring whole genome sequences of endophytic diazotrophs.Several representative new methods based on genomic data (ANI analysis,the maximal unique match index,core genome analysis,component vector method,gene flow analysis) were summarized.Based on the current research methods of phylogenetic evolution,research trends of the development and evolution of Endophytic Nitrogen Fixing Bacteria were summarized and prospected.The purpose of this paper is to make the phylogenetic evolution of Endophytic Nitrogen Fixing Bacteria more accuracy and reliability.
展开▼
