The taxonomy position and life cycles of culture-difficult fungi which associated with plants at various symbiosis extent are very completed and diverse. Most of these fungi can finish their life cycles when the other living things (such as plant, bacteria etc.) present. The non-obligate with no-vivo nutrition culture-difficult fungi are easier to be pure cultured than that of obligate with vivo nutrition one. On the basis of introducing taxonomy position, life cycle and symbiosis type of culture-difficult fungi associated with plants, the present author summarized the cultural characteristics and method for these fungi, as well as discussing the research trend and prospects, in order to provide new idea and basis for further investigating the pure culture of the fungi.%与植物共生难培养菌物的分类地位与生活史复杂多样,与植物共生程度各异.在其他一定种类生物(如植物、细菌等)存在的条件下,大多与植物共生难培养菌物能完成生活史,而且一些非专性非活体营养的共生菌物较专性活体营养的共生菌物更容易获得纯培养.在简要介绍与植物共生难培养菌物分类地位、生活史与共生类型的基础上,重点探讨了与植物共生难培养菌物的培养特性和培养方法,并讨论了该领域的研究动向与展望,旨在为当前和今后开展难培养菌物纯培养研究提供思路、依据和工作基础.
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