采用4种分离培养基对新疆乌鲁木齐市污水处理厂低温污水中的耐冷微生物进行分离筛选,共获得各种耐冷微生物154株,其中丝状真菌12株,酵母46株,放线菌6株,细菌90株.并对部分菌株的耐受性和产酶特性进行了研究,获得了耐4%NaCl的菌株44株,耐0.2%苯酚的16株,耐0.5%SDS的33株,具有淀粉酶活性的菌株40株,蛋白酶活性的30株,脂酶活性的27株.对其中60株细菌进行16S rRNA基因测序,序列比对分析表明,其分属13个属,其中菌株39与不动杆菌属中的标准菌株Acinetobacter lwoffii(DSM2403)同源性最近,为96.6%,极有可能为新种.证明低温污水中存在着丰富的耐冷微生物及种属多样性,且具有较好的耐受性及产酶特性,预示着低温污水可能是重要潜在的微生物资源库.%Psychrotrophs were isolated by using four media from low-temperature sewage of sewage treatment plant in Urumqi, Xinjiang. Totally, 154 strains were obtained including 12 filamentous fungi, 46 yeasts, 6 actinomycetes and 90 bacteria. The results of tolerance tests of the isolates to salt, phenol and SDS, and enzyme producing characters of amylase, proteinase and esterase were shown. Then 60 bacterial strains were chosen for 16S rRNA gene sequencing and analysis. The blasting results showed that the strains were assigned to 13 recognized genera , and the Strain 39 exhibited 96.6% similarity to Acinetobacter Iwof-fii(DSM2403), indicating that it might be a novel species. These results suggested that there were a lot of psychrotrophs and rich bacterial diversity in low-temperature sewage. In addition, which maybe an important and potential library of microbial resources.
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