目的:研究麦冬中高异黄酮类化合物及其生物活性.方法:利用多种色谱技术相结合的方法从麦冬中分离获得单体化合物,通过核磁共振方法确定化合物的结构.结果:从麦冬干燥块根中分离鉴定了10个化合物,分别为7-O-methyl-3'-hydroxy-3,9-dihydropunctatin(1),(3R)-3,7-dihydroxy-3-(4'-hydroxy-3'-methoxybenzyl)chroman-4-one(2),6-hydroxy-7-O-methyl-3,9-dihydropunctatin(3),7,4'-di-O-methyl-3'-hydroxy-3,9-dihydropunctatin(4),(3R)-3,7-dihydroxy-3-[furan-2'(5H)-one] chroman-4-one(5),(aS)-3,9,10-trihydroxy-7-hydro-6H-dibenz-[b,d]oxocin-7-one(6),(3R)-3-hydroxy-3-(4'-methoxybenzyl)-5,6,7-trimethoxychroman-4-one(7),(3R)-3-hydroxy-3-(4'-hydroxybenzyl)-5,6,7-trimethoxychroman-4-one(8),5-methoxy-6-methyl-7-hydroxy-8-aldehydo-3-(3',4'-methylenedioxy-benzyl) chroman-4-one(9),5,8,4'-trimethoxy-6-methyl-7,2'-dihydroxy-3-benzylchroman-4-one (10).体外细胞毒活性试验表明,化合物1、6、7具有抑制人肺癌细胞(A-549)增殖的活性,IC50值分别为10.1、13.5、8.0 μmol·L-1.结论:化合物1～8为首次从麦冬中分离得到.%Objective:To study the chemical constituents and bioactivities of the tuber of Ophiopogon japonicus.Methods:Single compounds were obtained by comprehensive chromatography technology.Structures were identified by nuclear magnetic resonance(NMR) technology.Results:Compounds isolated from the tuber were identified as 7-O-methyl-3'-hydroxy-3,9-dihydropunctatin (1),(3R)-3,7-dihydroxy-3-(4'-hydroxy-3'-methoxybenzyl) chroman-4-one (2),6-hydroxy-7-O-methyl-3,9-dihydropunctatin (3),7,4'-di-O-methyl-3'-hydroxy-3,9-dihydropunctatin (4),(3R)-3,7-dihydroxy-3-[furan-2'(5H)-one] chroman-4-one(5),(aS)-3,9,10-trihydroxy-7-hydro-6H-dibenz-[b,d] oxocin-7-one (6),(3R)-3-hydroxy-3-(4'-methoxybenzyl)-5,6,7-trimethoxychroman-4-one (7),(3R)-3-hydroxy-3-(4'-hydroxybenzyl)-5,6,7-trimethoxychroman-4-one (8),5-methoxy-6-methyl-7-hydroxy-8-aldehydo-3-(3',4'-methylenedioxybenzyl)chroman-4-one (9),and 5,8,4'-trimethoxy-6-methyl-7,2'-dihydroxy-3-benzylchroman-4-one (10),respectively.Compounds 1,6,and 7 exhibited cytotoxicity against A-549 with IC50 values of 10.1,13.5,8.0 μmol· L-1,respectively.Conclusion:Compounds 1-8 were obtained from the plant for the first time.
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