目的:通过性状和高效液相色谱指纹图谱对连作园参和新林地园参进行人参皂苷含量的比较,从而判断连作园参种植过程中使用的土壤改良技术是否具有抗人参连作障碍的作用.方法:采用有机试剂萃取法提取连作园参和新林地园参的人参总皂苷,再通过高效液相色谱仪测定两种人参的皂苷含量,最后使用中药色谱指纹图谱相似度评价系统A版对连作园参和新林地园参样品进行比对,通过分析计算得到上述样品的相似度.结果:连作园参中所含人参皂苷Rb1、Rg1、Re含量要高于新林地园参,指纹图谱显示2种园参与10种连作园参的高效液相色谱图的相似度具有一定差异性,连作园参没出现更严重的烧须、红皮现象.结论:连作园参的质量要优于新林地园参,证明该土壤改良技术具有一定的抗人参连作障碍的作用.%Objective:To evaluate a soil improving technology for ginseng continuous cropping obstacle through comparison of the character and HPLC fingerprint of continuous cropping garden and new woodland garden ginseng.Methods:The total saponins were extracted,and the contents of saponins in ginseng were determined by HPLC.Finally,the similarity evaluation system of traditional Chinese medicine chromatographic fingerprints was used to evaluate the total saponins of ginseng,and the parameters of the samples were compared with each other,and the similarity of the samples was calculated and analyzed.Results:The contents of ginsenoside Rb1,Rg1 and Re in continuous cropping garden ginseng were higher than that in new woodland garden ginseng deforestation,and the fingerprints showed that the similarity of high performance liquid chromatograms of 10 kinds of plants was different.There is no more serious burning beard,red skin phenomenon on continuous cropping garden ginseng.Conclusion:The quality of continuous cropping ginseng is better than that of new forestland ginseng,which proves that the soil improvement technology has certain anti continuous cropping effect.
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