To explore the effects of Dendrobium candidum polysaccharide on immunization,intestinal microbes and enzyme activities in mice with spleen deficiency constipation,and clear the role of polysaccharide in the treatment of spleen deficiency constipation.The mouse spleen deficiency constipation model was established by giving senna water decoction for 7 days,and then restricting their diet to make them hunger satiety disordered for 8 days.After the success of modeling,normal group and model group mice were treated with sterilized water by intragastric administration,the treatment groups were fed with D.candidum polysaccharide solution.Several indicators were analyzed including body weight,spleen coefficient,thymus coefficient,intestinal flora and enzyme activity.The results showed that there were significant differences in body weight,changing rate of body weight,spleen index,thymus index and the quantities of five culturable microorganisms (bacteria,Escherichia coli,Lactobacillus,Bifidobacterrium and Fungi) in the model group compared with these of normal group.After treatment,the body weight and changing rate of body weight increased significantly,higher than these of normal group;the spleen index and thymus index recovered to different degrees,closed to these of normal group;Escherichia coli recovered a lot,but far from the normal level;Lactobacillus and Bifidobacterium were significantly decreased,but the total number was significantly higher than the normal group;xylanase and protease were close to normal group,amylase was significantly increased,but cellulase significantly reduced.The results showed that D.candidum polysaccharides can promote body immunity,regulate the balance of intestinal microbiota and intestinal enzyme activity,optimize the intestinal environment and improve the symptoms of spleen constipation.%为了探讨铁皮石斛多糖对脾虚便秘小鼠免疫、肠道微生物及酶活性的影响,明确多糖对脾虚便秘的治疗作用,通过灌胃番泻叶水煎液7d后控制饮食,饥饱失常8d,建立小鼠脾虚便秘模型.造模成功后,正常组和模型组灌胃等量无菌水,治疗组灌胃铁皮石斛多糖溶液,分析小鼠体重、脾脏系数、胸腺系数、肠道菌群和酶活性的变化情况.结果表明,模型组的小鼠体重、体重变化率、脾脏指数、胸腺指数、五类可培养微生物(细菌总数、大肠杆菌、乳酸菌、双歧杆菌和真菌)数量与正常组比较差异显著(P＜0.01或P＜0.05).治疗完成后,小鼠体重和体重变化率显著增高,高于正常组(P＜0.05);小鼠脾脏指数和胸腺指数均有不同程度恢复,接近正常组(P＞0.05);大肠杆菌大量得到恢复,但远没有达到正常组水平(P＜0.01);乳酸菌和双歧杆菌明显地减少,但总数显著超过正常组(P＜0.01);木聚糖酶和蛋白酶接近正常组,淀粉酶显著增加,纤维素酶显著减少(P＜0.01).说明铁皮石斛多糖对增强机体免疫力、调控肠道微生态平衡和肠道酶活性方面有一定的促进作用,能优化肠道环境,改善脾虚便秘症状.
展开▼
