为探究伪原薯蓣皂苷对Ox-LDL诱导的内皮细胞COX-2表达的影响.体外培养人脐静脉内皮细胞(HUVEC),加入伪原薯蓣皂苷预处理细胞24 h,再以氧化低密度脂蛋白(Ox-LDL)诱导HUVEC细胞表达COX-2;采用Real-time PCR和western blot分别检测了COX-2的mRNA和蛋白表达水平,以及与COX-2炎症信号通路相关的激酶活性.结果显示,伪原薯蓣皂苷剂量依赖的下调COX-2的表达,同时抑制p38MAPK的激酶活性.这些结果表明,伪原薯蓣皂苷可能通过抑制TLR2/p38MAPK通路而抑制内皮细胞炎症介质COX-2的表达,提示其对内皮细胞的抗炎效应.%Pseudoprotodioscinis the main active compound of Di'aoXinXue Kang capsules.To explore the effect of pseudoprotodioscin on cyclooxygenase-2 (COX-2) expression induced by OX-LDL in human umbilical vein endothelial cells (HUVEC),HUVEC cells were cultured in vitro.After pretreatment with different concentrations of pseudoprotodioscin for 24 h,OX-LDL was added for the induction of COX-2 expression.The expressions of COX-2 mRNA was detected by Real-time PCR,and the expression of COX-2 protein and the phosphorylation of mitogen activated protein kinase (MAPK) associated with COX-2 inflammatory signaling pathway were determined by Western blot.Our results showed that pseudoprotodioscin can inhibit OX-LDL-induced COX-2 expression in HUVEC dose-dependently,and inhibit TLR2 expression and p38MAPK activity.These results indicated that pseudoprodioscininhibited OX-LDL-induced COX-2 expression possibly via affecting the TLR2 / p38MAPK pathway,suggesting its anti-inflammatory effect on vein endothelial cells.
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