采用DEAE-52柱层析及Sephadex G-200凝胶柱层析从羊肚菌多糖中分离纯化出两种水溶性多糖(MEP1-1和MEP 2-3).研究了其分子量、单糖组成、体外抗氧化活性及抗肿瘤活性.结果表明,MEP1-1和MEP2-3分子量分别为1.37×105 Da和1.15×104 Da.红外光谱显示MEP 1-1,MEP 2-3含有少量β糖苷键.MEP 1-1和MEP2-3抗氧化活性均高于粗多糖,MEP2-3具有较高的羟基自由基活性,而MEP1-1具有较强的清除超氧阴离子自由基活性.MEP 1-1比MEP2-3表现出更高的抑制HeLa活性,IC50值为29 μg/mL.而MEP2-3表现出更高的抑制A549活性,72 h的IC50值为81 μg/mL.%Two water-soluble polysaccharides (MEP1-1 and MEP2-3) were extracted and purified using chromatography on DEAE-52 and Sephadex G-200 column from Morchella esculenta.Their chemical composition and in vitro antioxidant and antitumor activities were investigated.The results suggested that the molecular weights of MEP 1-1 and MEP 2-3 were approximately 1.37 × 105 Da and 1.15 × 104 Da,respectively.Characteristic bands in FT-IR spectra indicated both MEP1-1 and MEP2-3 had a small amount ofβ-glycosidic bond.Antioxidant activities of MEP1-1 and MEP2-3 were both higher than crude MEP.MEP2-3 had higher antioxidant activities on hydroxyl radicals than MEP1-1 while MEP1-1 had higher antioxidant activities on superoxide radicals than MEP2-3.MEP 1-1 fraction appeared higher anticancer activity against HeLa cells than MEP2-3 fraction,with IC50 values of 29 μg/mL.MEP2-3 fraction showed higher anticancer activity against A549 cells than MEP 1-1 fraction,with IC50 values of 81 μg/mL at 72 h.It could be explored as a potential adjuvant against cancer used in the health food and pharmaceutical therapy.
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