本文探讨了罗氏乳杆菌DSM122460无细胞上清培养液(Cell-Free Supernatant,CFS)移除胆固醇的能力.采用邻苯二甲醛法测定DSM122460和对照菌株ST-Ⅲ发酵过程中及其CFS对胆固醇的移除能力,并研究不同CFS浓度下的移除能力.并采用HPLC法测定CFS对照、热处理组和pH7.0组的胆盐水解酶活力,同时测定其移除胆固醇能力.结果显示,DSM122460不仅在发酵过程中具有较高的移除胆固醇能力,其CFS也表现出较高的移除能力,CFS中含有除胆盐水解酶以外的可移除胆固醇的蛋白类成分.这提示可能存在一种乳酸菌移除胆固醇的新机制.%The cholesterol removal ability of cell-free supernatant (CFS) produced by growth of Lactobacllus reuteri DSM122460 in MRS broth was investigated. The cholesterol removal ability of L reuteri train DSM122460 as well as L. plantarum strain ST-III during growth in MRS and the resulted CFS was assayed by o-phthalaldehyde method,respectively. In order to elucite the correlation between BSH activity and the cholesterol removal ability of the LactobacUlus strains, BSH roles in cholesterol removal were measured by heat treatment and pH adjustment. The remaining BSH activities after various treatments were measured by HPLC. The results showed that L reuteri strain DSM122460 not only had a high a-bility to remove cholesterol during its growth in MRS broth, it aslo demonstrated high cholesterol reduction activity in the CFS. Beside the BSH.it was speculated there may be another effective component in the CFS with the ability to remove cholesterol. It suggested that there might be a new mechanism underlying cholesterol reduction by lactic acid bacteria.
展开▼
