研究冬葵果多糖(Fructus Malvae polysaccharides,FMP)对氧自由基的清除作用及对脂质过氧化的抑制作用.采用分光光度法测定FMP清除Fenton体系产生的羟自由基(·OH)、邻苯三酚自氧化体系产生的超氧阴离子自由基(O2-)的能力及评价对Fe(Ⅱ)-H2O2体系(·OH)诱导的脂质过氧化终产物丙二醛(MDA)的抑制作用.结果显示,在化学模拟体系中,冬葵果多糖对·OH具有很强的清除作用,与Vc比较达到显著水平(P＜0.01),对O2-的清除能力与Vc相当.在体外,多糖浓度达到1.72 mg/mL可明显降低MDA的含量,与空白液比较达到显著水平(P＜0.01).以上结果表明,FMP具有明显的抗氧化活性.%Both the oxygen free radical scavenging activity and the inhibitory action to lipid peroxidan'on of Fructus Mal-vae polysaccharides ( FMP) were studied. The antioxidant effects of FMP on hydroxyl ( · OH) produced by Fenton reaction and super-oxide radical (O2-) produced by the oxidation of pyrogallol were determined by the spectrophotometer method. Its inhibition effect on MDA induced by Fe ( Ⅱ)·H2O2 system (·OH) was also evaluated by the above method. The results showed that FMP had stronger scavenging effect on ·OH in some chemically modified systems with a significant difference (P < 0.01) compared with Vc, and its scavenging activity on O2- was equivalent to that of Vc. In vitro,when the concentration of polysaccharides up to 1.72 mol/mL,it obviously reduced the content of MDA,there was a significant difference (P<0.01) compared with blank solution. Above experimental results showed that FMP has the strong antioxidation activity in vitro which relates to remove oxygen free radical.
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