In order to explore the efficient and low contaminated extraction process of vindoline, catharanthine, vincristine and vinblastine from Catharanthus roseus, comprehensive optimization of condition in ultrasonic-methanol method were studied. On the basis of single factor experiments, orthogonal test was carried out to study. The optimum parameters for obtaining vindoline and catharanthine were determined as follows:methanol as solvent was used for 180 min,which pH value was 4.0. The ratio of raw material to solvent was 1: 15 (w/v), the extraction temperature was 45 ℃, and extraction frequency was 59 KHz. The yield of vindoline and catharanthine in the conditions was 0.164％ and 0.081％ ,respectively.%为探索高效、低污染的长春花中文多灵、长春质碱、长春新碱和长春碱的提取工艺,进行了超声波-甲醇法提取工艺条件的优化研究.结果表明:在单因素试验基础上,通过正交试验表明,超声波-甲醇法提取文多灵和长春质碱的最佳工艺条件为:提取溶剂:甲醇(pH4.0);料液比:1∶15;提取温度:45℃,提取时间:180 min,提取频率:59 KHz;文多灵和长春质碱平均得率为0.16%,0.08%.
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