Objective: To investigate the effect of thymic stromal lymphopoietin (TSLP) on the differentiation of intrathymic CD4+CD25- T cells to CD4+CD25+Foxp3+ regulatory T cells in myasthenia gravis (MG) patients with abnormal thymus gland. Methods: Intrathymic CD4+CD25-T cells and dendritic cells isolated by immunomagnetic beads on MiniMACS device were co-cultured with different concentrations of TSLP (0.1, 1, 10, 20 ng/mL) for 7 days. The ratio of CD4+CD25+Foxp3+Treg/CD4+T cells was tested by flow cytometry. The expression of Foxp3 mRNA and protein was measured by RT-PCR and Western blot respectively. The concentration of serum IL-10 was detected by ELISA. Results: The ratio of CD4+CD25+Foxp3+Treg/CD4+T cell, the expression of Foxp3 mRNA and protein, and the level of IL-10 was increased significantly in groups with 10 ng/mL and 20 ng/mL TSLP compared with those in the PBS control group (P<0.05). Conclusion: TSLP may induce the differentiation of intrathymic CD4+CD25 -T cells to CD4+CD25+Foxp3+regulatory T cells in MG patients with abnormal thymus gland and up-regulate the expression of Foxp3.%目的：探索胸腺基质淋巴细胞生成素（TSLP）对重症肌无力（MG）合并胸腺异常患者胸腺 CD4+CD25－T细胞诱导生成 CD4+CD25+Foxp3+调节性 T （Treg）细胞的研究。方法：以磁珠分选的方法得到胸腺CD4+CD25－T 细胞及树突状细胞（DC）细胞，通过不同浓度（0.1、1、10、20 ng/mL）的 TSLP 活化 DC 后诱导CD4+CD25－T 细胞向 CD4+CD25+Foxp3+Treg 细胞分化，以流式细胞术检测 CD4+CD25+Foxp3+Treg 细胞/CD4+T 细胞比率；RT-PCR 检测 Foxp3 mRNA 表达；Western blot 法检测上调的 CD4+CD25+Foxp3+Treg 细胞的Foxp3蛋白表达；ELSIA 法检测培养上清 IL-10的含量。结果：10 ng/mL TSLP 组、20 ng/mL TSLP 组经诱导后CD4+CD25+Foxp3+Treg 细胞/CD4+T 细胞比例均明显升高，Foxp3的表达水平也相应增加，IL-10水平升高，与PBS 对照组相比差异有统计学意义（P<0.05）。结论：TSLP 可以诱导 MG 合并胸腺异常患者胸腺 CD4+CD25－T 细胞向 CD4+CD25+Foxp3+Treg 细胞分化，并促进 Foxp3的表达。
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