目的：观察硫化氢(H2S)及其合成酶胱硫醚⁃β⁃合成酶（CBS）、胱硫醚⁃γ⁃裂解酶（CSE）和3⁃巯基丙酮酸硫转移酶（MPST）在人成骨细胞株及人成骨肉瘤细胞系中的表达。方法我科于2013年6月至2014年12月运用免疫组化染色和蛋白质印迹方法在人永久性成骨细胞株hFOB1.19，成骨肉瘤细胞株Saos⁃2、MG⁃63和U⁃2 OS中分别检测CBS、CSE和MPST基因表达量、蛋白含量；通过敏感硫电极法检测H2S在正常成骨组织和骨肉瘤细胞中的含量。分别比较H2S及其合成酶在各组之间的差异，初步探讨H2S及其合成酶与不同成骨肉瘤细胞之间的关系。结果在成骨肉瘤标本和细胞中CBS、CSE和MPST基因的较高表达；成骨肉瘤组织与成骨细胞来源的正常和恶性肿瘤细胞CBS、CSE和MPST基因表达的蛋白水平以及H2S产生率不同，其中成骨肉瘤细胞株U⁃2 OS表达水平最高。结论内源性H2S及它的合成酶CBS、CSE和MPST存在于人恶性成骨肉瘤组织和细胞中，高水平的H2S及其生物合成物质或许能成为成骨肉瘤治疗的新靶点。%Objective To investigate the expression levels of endogenous hydrogen sulfide (H2S) and its synthases cystathionineβ⁃synthase (CBS), cystatbionineγ⁃lyase (CSE) and 3⁃mercaptopyruvate sulfurtrans⁃ferase (MPST) in osteosarcoma cells. Methods From June 2013 to December 2014, immunohistochemistry and Western blotting were used to detect CBS, CSE and MPST mRNA and protein expression in hFOB1.19, Saos⁃2, MG⁃63 and U⁃2 OS cells, and H2S was determined in normal tissue samples and osteosarcoma cells. Re⁃sults There was a higher expression of CBS, CSE and MPST in osteosarcoma samples and cells. The osteosar⁃coma tissue and osteoblast⁃derived normal and malignant cell lines presented different protein levels of CBS, CSE and MPST, among which osteosarcoma cancer cells U⁃2 OS expressed highest. Conclusion H2S and its synthases CBS, CSE, and MPST exist in the human malignant osteoblastic tissues and cells. These findings im⁃ply that high levels of H2S and its biosynthesis may be a new target for osteosarcoma treatment.
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