The seed germination experiment is used to study the different concentrations (0,1 and 2,10 mL/dish) and the different ramie varieties rhizosphere soil water extract allelopathic effect on the model plant Arabidopsis thaliana seedlings,and relative real-time quantitative PCR (QPCR) technology was applied to probe into the YUC1 (AT4G32540) gene expression related to auxin biosynthesis after treatment.Results showed that different concentration of water extracts from rhizosphere soil of ramie had certain allelopathy on the Arabidopsis thaliana,and the allelopathy enhances with the increase of concentration.The malondialdehyde (MDA) content of Arabidopsis thaliana seedlings rises with the increase in concentration of extract under the rhizosphere ZhongZhu 1 treatment.Compared with control,malondialdehyde (MDA) content of seedlings reduced in lower concentration and increased in higher concentration of extract under the rhizosphere Xiangzhu 7 treatment.The superoxide dismutase (SOD) activity decreased with the rising of treatment concentration.QPCR detection results showed that the gene expressive quantity of YUCI reduced with the increase of water extract concentration under the processing of two ramie varieties.In conclusion,with the increase of the extract concentration,ramie has an increase Allelopathy on Arabidopsis growth.%通过种子萌发实验研究了不同浓度(0、1、2、10 mL/皿)不同苎麻品种根际土壤水浸液对模式植物拟南芥幼苗生长的化感效应,并运用相对实时定量PCR (QPCR)技术从基因水平探讨了不同浓度根际土壤水浸提液处理后与拟南芥生长素合成相关基因YUC1(AT4G32540)的表达.结果表明,随着苎麻根际土壤水浸液浓度的升高,拟南芥幼苗在中苎1号根际土壤处理下其根长与对照相比均呈现出抑制效应,在湘苎7号根际土壤处理下其根长与对照相比呈现出低促高抑现象.在中苎1号根际土壤处理下拟南芥幼苗丙二醛(MDA)含量随着苎麻根际土壤水浸提液浓度升高而上升,在湘苎7号根际土壤处理下拟南芥幼苗丙二醛(MDA)含量在低浓度时,与对照相比降低,当浓度升高,其含量呈现出升高趋势.在不同浓度根际土壤水浸提液处理下,拟南芥幼苗的过氧化物歧化酶(SOD)活性均表现为随处理浓度升高,SOD活性下降,且在中苎1号处理下,下降幅度较湘苎7号大.QPCR检测结果表明,2个苎麻品种处理下YUC1其基因的表达量随水浸提液浓度增加均降低,且中苎1号下降幅度较湘苎7号大.可见,随浸提液浓度升高,苎麻对拟南芥生长的化感胁迫性增强.
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机译：Allelopathic Effects of Aqueous Extracts of Different Tissues and Rhizosphere Root of Adzuki Bean on Growth of Wheat Seedlings红小豆不同组织及根际土壤水浸提液对小麦幼苗生长的影响
