In the study,the conventional paraffin method was applied to explore the period and process of pollen aborsion in dominant nuclear sterile flax using male fertile and male sterile flax separa-ted from the dominant nuclear sterile flax line as material. Temporal and spatial expression of male sterili-ty related gene MS2-F in flax anther was studied. Digoxin labeled MS2 -F specific cRNA probe was synthesized in vitro using T7 and SP6 primers,and was hybridized with flax anther slice. Strong hybrid-ization signal was observed in the tapetum from the period of tetrad to the early period of microspore de-velopment,and no hybridization signal was observed during pre -tetrad period,proving that MS2 -F was specifically expressed in tapetum during the period of tetrad and microspore early development.%本研究采用常规石蜡切片方法和原位杂交技术，以雄性可育株和雄性不育株亚麻为材料，利用T7和S P6引物对体外转录合成地高辛标记的MS2－F基因特异c RN A探针，并与亚麻花蕾切片进行原位杂交，观察MS2－F mRNA在亚麻花蕾内的时空表达信号。结果发现：亚麻MS2－F在四分体时期和小孢子发育早期的花蕾绒毡层中有杂交信号，而在四分体时期之前的绒毡层细胞中，没有MS2－F杂交信号；该基因从四分体时期开始到形成小孢子早期的绒毡层细胞中表达，进一步证明了该基因在亚麻花蕾发育过程中起着非常重要的作用，但是否具有关键性的作用需要进一步的深入研究。
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