Objective To investigate the influence of activity of superoxide dismutase (SOD),level of malondialdehyde(MDA) in the serum,and activity of creatine kinase(CK) in musclar tissue in the forced posture rabbits at resonance. Methods Animals (75 New Zealand rabbits) were randomly divided into normal control group,modeling control group,4 Hz vibration group,the 5 Hz vibration group and 6 Hz vibration group,n=15 for each. Peripheral blood was taken from 5 rabbits at each group at the end of the 2 ,4 and 6 weeks separately. After the animals were killed,the psoas about 0.2 g were taken. Then the activity of the superoxide dismutase (SOD),the content of the malondialdehyde (MDA) in the serum,and the activity of creatine kinase in the homogenate were detected. Results The activity of SOD of different vibration group was less than normal control group at different time (P<0.05),and with the development of the time the activity of SOD of different group was less and less(P<0.05);the content of the MDA of different vibration group was more than normal control group at different time(P<0.05),and with the development of the time the content of the MDA of different group was more and more (P<0.05);the activity of the CK of different vibration group was more than normal control group at different time (P<0.05),and with the development of the time the activity of the CK of different group was more and more(P<0.05). Conclusion Forced posture and the resonance can cause the change of the activity of SOD, the content of the MDA in the serum and the activity of the CK in muscles of low back ,and related to time. The influence on the serum markers of muscles of resonance at the frequency of 4Hz,acceleration of 1.0g is most obvious. A negative correlation is found between the content of MDA and the activity of SOD. The extent of damage of the muscles is correlated positively with the content of the MDA.%目的：探讨强迫体位下谐振对兔血清超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量和肌肉组织中肌酸激酶（CK）活性的影响。方法将实验动物随机分为正常对照组、造模对照组、4 Hz振动组、5 Hz振动组、6 Hz振动组。分别于第2、4、6周清晨空腹随机取5只进行耳缘静脉取血，测定血清SOD的活性和MDA的含量，随后取腰大肌约0．2 g，组织匀浆后测定CK的活性。结果不同时间点各振动组SOD活性低于正常对照组（P＜0．05）；各组SOD活性随时间延长逐渐降低（P＜0．05）。不同时间点各振动组MDA含量高于正常对照组（P＜0．05）；各组MDA含量随时间延长逐渐升高（P＜0．05）。不同时间点造模对照组和各振动组CK活性高于正常照组（P＜0．05），各组CK活性随时间延长逐渐升高（P＜0．05）。结论强迫体位和谐振对血清SOD活性、MDA含量及肌肉中CK活性的影响与时间长短密切相关，全身振动以频率4 Hz、加速度1．0 g的振动影响最为明显。强迫体位下谐振兔血清SOD活性和MDA含量变化呈负相关，肌肉的损伤程度与血清MDA的含量呈正相关。
展开▼
